PENGARUH THIDIAZURON DAN NAPHTALENE ACETIC ACID UNTUK INDUKSI EMBRIOGENESIS SOMATIK DARI DAUN ANGGREK Phalaenopsis “Sogo Vivien”
Abstract
Phalaenopsis “Sogo Alice” X Phalaenopsis “Zuma‟s Pixie” dengan karakteristik morfologi
berukuran mini serta memiliki bunga serta percabangan infloresensia yang banyak, sehingga
dapat digunakan sebagai tanaman hias dalam pot. Embriogenesis somatik merupakan teknik
mikropropagasi in vitro yang dapat menghasilkan anakan yang seragam dalam jumlah besar.
Induksi embriogenesis somatik pada anggrek P. “Sogo Vivien” dapat dilakukan melalui
perlakuan zat pengatur tumbuh Thidiazuron (TDZ) (0,5 dan 10 mg/l) yang dikombinasikan
dengan Naphtalene Acetic Acid (NAA) 1 mg/l. Eksplan yang digunakan berupa daun dari
seedling in vitro anggrek P. “Sogo Vivien”. Hasil penelitian menunjukkan bahwa TDZ 10 mg/l
yang dikombinasikan dengan NAA 1 mg/l mampu menginduksi pembentukan embrio somatik
secara langsung pada eksplan daun anggrek P. “Sogo Vivien” in vitro. Efisiensi pembentukan
embrio somatik yang diperoleh adalah 5,7% dengan jumlah embrio somatik yang terbentuk
adalah 57 embrio dari 1 eksplan daun. Hasil penelitian ini menunjukkan bahwa embrio somatik
dapat diinduksi pada eksplan daun anggrek P. “Sogo Vivien”dengan menggunakan TDZ dan
NAA
Kata kunci : anggrek, embriogenesis somatik, thidiazuron, naphthalene-acetic-acid
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UntitledReferences
Arnold, S., Sabala, I., Bozhkov, P.,
Dyachok, J., and Filonova, L. 2002.
Development pathways of somatic
embryogenesis. Plant Cell, Tissue &
Organ Culture 69: 233-249.
Chen, J.T., Chang, C., and Chang, W.C.
Direct somatic embryogenesis
on leaf explants of Oncidium „Gower
Ramsey‟ and subsequent plant
regeneration. Plant Cell Report 19:
–149
_____________________________. 2004.
Plant regeneration through shoot bud
formation from leaf cultures of
Paphiopedilum orchids. Plant Cell,
Tissue & Organ Culture 76: 11–15.
Chen, J.T. and Chang,W.C., 2006. Direct
somatic embryogenesis and plant
regeneration from leaf explants of
Phalaenopsis amabilis. Biologia
Plantarum 50: 169–173.
Chugh, S., Guha, S., and Rao, I.U. 2009.
Micropropagation of orchids: A
review on the potential of different
explants. Scientia Horticulturae 122:
-520.
Griesbach, R.J. 2002. Development of
Phalaenopsis orchids for the mass
market. In: Jainick, J. and Whipkey,
A (Eds.) Trends in New Crops and
New Uses. ASHS Press. Alexandria.
VA.
Indrianto, A. 2003. Bahan Ajar Kultur
Jaringan Tumbuhan. Fakultas
Biologi. UGM. Yogyakarta.
Islam, M.O., Ichihashi, S., and Matsui, S.
Control of groeth and
development of protocorm like body
from callus by carbon sources in
Phalaenopsis. Plant Biotechnology
(4): 183-187.
Jimenez, V. M. 2001. Regulation of in vitro
somatic embryogenesis with
emphasis on the role of endogenous
hormones. Revista Brasileira de
Fisiologia Vegetal 13(2): 196-223.
Karami, O., Aghavaisi, B., and Pour, A.M.
Molecular aspect of somaticto-embryogenic transition in plants.
Journal of Chemical Biology 2(4):
-190.
Khoddamzadeh, A. A., Sinniah, U. R.,
Kadir, M. A., Kadzimin, S. B.,
Mahmood, M., and Sreeramanan, S.
In vitro induction and
proliferation of protocorm-like
bodies (PLBs) from leaf segments of
Phalaenopsis bellina (Rchb.f.)
Christenson. Plant Growth
Regulation 65: 381-387.
Kosir, P., Suzana, S., and Zlata, L. 2004.
Direct shoot regeneration from nodes
of Phalaenopsis orchids. Acta
Agriculturae Slovenica 83:233–242.
Kuo, H.L., Chen, J.T., and Chang, W.C.
Efficient plant regeneration
through direct somatic
embryogenesis from leaf explants of
Phalaenopsis „Little Steve‟. In Vitro
Cellular and Development Biology
Plant 41: 453–456.
Martin, K.P. and Madassery, J.P. 2006.
Rapid in vitro propagation of
Dendrobium hybrids through direct
shoot formation from foliar explants
and protocorm like bodies. Scientia
Horticulturae 108: 95–99.
Park, S.Y., Yeung, E.C., Chakrabarty, D.,
and Paek, K.Y. 2002. An efficient
direct induction of protocorm-like
bodies from leaf subepidermal cells
of Doritaenopsis hybrid using thinsection culture. Plant Cell Reports
:46-51
Tang, C. and Chen, W. 2007. Breeding and
development of new varieties in
Phalaenopsis. pp: 1-22. In: Chen, W.
and Chen, H. (Eds.). Orchid
Biotechnology. World Scientific
Publishing. Singapore.
Tokuhara, K. and Mii, M. 2001. Induction of
embryogenic callus and cell
suspension culture from shoot tips
excised from flower stalk buds of
Phalaenopsis (Orchidaceae). In Vitro
Cellular and Developmental Biology
Plant 37(4) : 457-461
Utami, E.S.W., Soemardi, I., Taryono dan
Semiarti, E. 2007. Embriogenesis
somatik anggrek bulan Phalaenopsis
amabilis (L.) Bl. : Struktur dan pola
perkembangan. Berkala Penelitian
Hayati 13: 33-38
Vendrame, W.A., Maguire, I., and
Carvalho, V.S. 2007. In Vitro
propagation and plantlet regeneration
from Doritaenopsis Purple Gem
„Ching Hua‟ Flower Explants.
HortScience 42(5): 1256 – 1258.
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